Difference between revisions of "Last news on SAMPLING"

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The diversity of ''Caenorhabditis'' is just starting to be uncovered, and we likely have only scratched the surface.  
 
The diversity of ''Caenorhabditis'' is just starting to be uncovered, and we likely have only scratched the surface.  
 
And remember that the cash and naming [http://forums.wormbase.org/index.php?topic=308.0 prize] for the ''C. elegans'' sister species is - unfortunately - still not claimed. So please SAMPLE and share!
 
And remember that the cash and naming [http://forums.wormbase.org/index.php?topic=308.0 prize] for the ''C. elegans'' sister species is - unfortunately - still not claimed. So please SAMPLE and share!
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Please submit your wild strain information to Wormbase through http://tazendra.caltech.edu/~azurebrd/cgi-bin/forms/wild_isolate.cgi
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or e-mail the information in a table to genenames@wormbase.org, copying to david.fitch@nyu.edu and wormstrain@biologie.ens.fr (Felix lab) for integration in the Rhabditina database (http://128.122.60.136/fmi/iwp/cgi?-db=RhabditinaDB&-loadframes) and the world map (to be announced).
 
For methods, see: [http://www.wormbook.org/chapters/www_nematodeisolation/nematodeisolation.html WormMethod chapter by Barrière and Félix], [http://groups.molbiosci.northwestern.edu/andersen/Collections.html Andersen lab].
 
For methods, see: [http://www.wormbook.org/chapters/www_nematodeisolation/nematodeisolation.html WormMethod chapter by Barrière and Félix], [http://groups.molbiosci.northwestern.edu/andersen/Collections.html Andersen lab].
  
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''Félix lab''
 
''Félix lab''
  
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2014:
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- C. nigoni from Yucatan, Mexico
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2013:
 
The fall 2013 was very rainy and busy, with sampling of lots of ''C. elegans'', ''C. briggsae'' and associated organisms from France by Lise Frézal, Sarah Marsh and MAF. Be prepared to pronounce "Crouy-sur-Ourcq" as a new location. The Orsay virus was finally found again by Lise in Ivry near Paris. Sarah established a collection of bacteria isolated from ''C. elegans'' and ''C. briggsae'', presumably most from their guts. Marie-Anne isolated an oomycete infecting ''C. elegans'', identified by 18S as a ''Myzocytiopsis'' sp. (for those who like her have no clue what oomycetes are, they are related to brown algae and diatoms).  
 
The fall 2013 was very rainy and busy, with sampling of lots of ''C. elegans'', ''C. briggsae'' and associated organisms from France by Lise Frézal, Sarah Marsh and MAF. Be prepared to pronounce "Crouy-sur-Ourcq" as a new location. The Orsay virus was finally found again by Lise in Ivry near Paris. Sarah established a collection of bacteria isolated from ''C. elegans'' and ''C. briggsae'', presumably most from their guts. Marie-Anne isolated an oomycete infecting ''C. elegans'', identified by 18S as a ''Myzocytiopsis'' sp. (for those who like her have no clue what oomycetes are, they are related to brown algae and diatoms).  
  

Revision as of 14:32, 6 September 2014

The diversity of Caenorhabditis is just starting to be uncovered, and we likely have only scratched the surface. And remember that the cash and naming prize for the C. elegans sister species is - unfortunately - still not claimed. So please SAMPLE and share! Please submit your wild strain information to Wormbase through http://tazendra.caltech.edu/~azurebrd/cgi-bin/forms/wild_isolate.cgi or e-mail the information in a table to genenames@wormbase.org, copying to david.fitch@nyu.edu and wormstrain@biologie.ens.fr (Felix lab) for integration in the Rhabditina database (http://128.122.60.136/fmi/iwp/cgi?-db=RhabditinaDB&-loadframes) and the world map (to be announced). For methods, see: WormMethod chapter by Barrière and Félix, Andersen lab.


Félix lab

2014: - C. nigoni from Yucatan, Mexico


2013: The fall 2013 was very rainy and busy, with sampling of lots of C. elegans, C. briggsae and associated organisms from France by Lise Frézal, Sarah Marsh and MAF. Be prepared to pronounce "Crouy-sur-Ourcq" as a new location. The Orsay virus was finally found again by Lise in Ivry near Paris. Sarah established a collection of bacteria isolated from C. elegans and C. briggsae, presumably most from their guts. Marie-Anne isolated an oomycete infecting C. elegans, identified by 18S as a Myzocytiopsis sp. (for those who like her have no clue what oomycetes are, they are related to brown algae and diatoms).

And thanks to many samplers, in the last year we isolated:

- a new species C. sp. 31 from Peru, with a beautiful male tail, as well as some C. briggsae and C. elegans from Peru

- C. briggsae from Mayotte

- a sp. 9 from Sao Tome, extending the Afro-Asian equatorial distribution of this species (from W to E: Sao Tome, Congo, Seychelles, Kerala, Singapore)

- a second C. elegans isolate from Greece

- a new collection of C. elegans from the botanical garden of Lisbon, Portugal

- a sp. 11 from Costa Rica

- C. elegans from Kerala, South of India!

- C. remanei from Strasbourg ... and from near Paris (thanks to Christian Braendle's prior finding)

- and finally sp. 6 again (after Michael Ailion first found it in Portugal) near Paris and in Ireland, all in rotting apples.

Conclusion: we are far from having sampled the Paris region to saturation, finding two more species this year, adding up to five (Cel, Cbr, Cre, spp. 13 and 6). Nouragues in French Guiana still holds the species diversity record with six species in a smaller area.

Tell us if you are interested in some of these isolates (here is our current collection of isolates, not yet including the most recent, nor our collection of C. elegans kept as potential heterozygotes by freezing the whole progeny of each wild P0 individual).


Braendle lab

Sampling news 2013-14

- Châtenay-Malabry, France (near Paris): C. elegans, C. briggsae and C. remanei

- Naples, Italy: C. elegans, C. briggsae

- Lisbon, Portugal: C. elegans

- Cadiz, Spain: C. elegans

- Cordoba, Spain: C. elegans, C. sp. 6

- Taiwan: C. briggsae, C. sp. 11, C. sp. 5 (sampling by Charlie Gosse - merci!)


Cutter lab

Sampling news 2014

- Koffler Scientific Reserve (near Toronto, Canada): C. remanei found commonly in May on isopods (Porcellio, Oniscus), but very rare June-August

- French Guiana, Parare station in Nouragues reserve (with C. Braendle): 200 opportunistic samples collected, 300 orange bait samples collected, preliminary looks suggest approx 120 strains of Caenorhabditis (both hermaphrodite and dioecious)