Difference between revisions of "GENETIC TOOLS, TRANSGENESIS, RNAi in different species"

From Nematode Evolution Community
Jump to navigation Jump to search
Line 11: Line 11:
 
  RNAi
 
  RNAi
 
RNAi susceptibility is a fast-evolving trait. External administration of dsRNA (synthetic dsRNA, or ds-RNA producing bacterial feeding) does not work in all species: [http://www.pnas.org/content/104/25/10565.long  Winston et al. 2007], [http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029811 Nuez & Félix 2012]. Efficiency also varies between tissues, including the germ line, even in ''C. elegans'': [http://www.wormbase.org/resources/paper/WBPaper00005458#0--10 Tijsterman et al. 2002]; [http://www.g3journal.org/content/3/6/941.long Pollard & Rockman 2013].
 
RNAi susceptibility is a fast-evolving trait. External administration of dsRNA (synthetic dsRNA, or ds-RNA producing bacterial feeding) does not work in all species: [http://www.pnas.org/content/104/25/10565.long  Winston et al. 2007], [http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029811 Nuez & Félix 2012]. Efficiency also varies between tissues, including the germ line, even in ''C. elegans'': [http://www.wormbase.org/resources/paper/WBPaper00005458#0--10 Tijsterman et al. 2002]; [http://www.g3journal.org/content/3/6/941.long Pollard & Rockman 2013].
 +
 +
 +
 +
GENETIC EDITING
 +
The TALEN approach works in ''C. briggsae'' [http://dx.doi.org/10.1534/genetics.113.155382  Lo et al. 2013],[http://dx.doi.org/10.1126/science.1207773 Wood et al. 2011], [http://dx.doi.org/10.1093/molbev/mst213 Wei et al. 2013]
 +
 +
The CRISPR/Cas9 method should work in ''C. briggsae'' and other species...

Revision as of 15:19, 13 January 2014


TRANSGENESIS

Microinjection and extra-chromosomal (Ex) array formation works seems to work in most Caenorhabditis species: Table 1 in Nuez & Félix 2012 . However, even in C. briggsae, the formation of Ex arrays is less efficient than in C. elegans and mosaic silencing can be observed in somatic tissues.

Bombardment requires a positive selection marker: unc-119 mutants are available in C. briggsae, Zhao et al. 2010; dual antibiotic selection was implemented in Cel CB4856, Cbr, Cre, Cbn: Semple et al. 2012.


RNAi

RNAi susceptibility is a fast-evolving trait. External administration of dsRNA (synthetic dsRNA, or ds-RNA producing bacterial feeding) does not work in all species: Winston et al. 2007, Nuez & Félix 2012. Efficiency also varies between tissues, including the germ line, even in C. elegans: Tijsterman et al. 2002; Pollard & Rockman 2013.


GENETIC EDITING

The TALEN approach works in C. briggsae Lo et al. 2013,Wood et al. 2011, Wei et al. 2013

The CRISPR/Cas9 method should work in C. briggsae and other species...