Difference between revisions of "GENETIC TOOLS, TRANSGENESIS, RNAi in different species"
(Created page with "GENETIC TOOLS, TRANSGENESIS, RNAi in different ''Caenorhabditis'' species Transgenesis [http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029811 Table 1 in Nue...") |
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| − | + | TRANSGENESIS | |
| − | [http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029811 Table 1 in Nuez & Félix 2012 ] | + | Microinjection and extra-chromosomal (''Ex'') array formation works seems to work in most ''Caenorhabditis'' species: [http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029811 Table 1 in Nuez & Félix 2012 ]. However, even in ''C. briggsae'', the formation of ''Ex'' arrays is less efficient than in ''C. elegans'' and mosaic silencing can be observed in somatic tissues. |
| − | + | Bombardment requires a positive selection marker: availability of ''unc-119'' mutants in [http://www.genetics.org/content/184/3/853.full ''C. briggsae'', Zhao et al. 2010] | |
| − | + | Dual antibiotic selection in Cel (N2, CB4856), Cbr, Cre, Cbn: [http://www.nature.com/nmeth/journal/v9/n2/full/nmeth.1864.html?WT.ec_id=NMETH-201202 Semple et al. 2012] | |
RNAi | RNAi | ||
| − | + | RNAi susceptibility is a fast-evolving trait. External administration of dsRNA (synthetic dsRNA, or ds-RNA producing bacterial feeding) does not work in all species: [http://www.pnas.org/content/104/25/10565.long Winston et al. 2007], [http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029811 Nuez & Félix 2012]. Efficiency also varies between tissues, including the germ line, even in ''C. elegans'': [http://www.wormbase.org/resources/paper/WBPaper00005458#0--10 Tijsterman et al. 2002]; [http://www.g3journal.org/content/3/6/941.long Pollard & Rockman 2013]. | |
Revision as of 17:40, 30 December 2013
TRANSGENESIS
Microinjection and extra-chromosomal (Ex) array formation works seems to work in most Caenorhabditis species: Table 1 in Nuez & Félix 2012 . However, even in C. briggsae, the formation of Ex arrays is less efficient than in C. elegans and mosaic silencing can be observed in somatic tissues.
Bombardment requires a positive selection marker: availability of unc-119 mutants in C. briggsae, Zhao et al. 2010
Dual antibiotic selection in Cel (N2, CB4856), Cbr, Cre, Cbn: Semple et al. 2012
RNAi
RNAi susceptibility is a fast-evolving trait. External administration of dsRNA (synthetic dsRNA, or ds-RNA producing bacterial feeding) does not work in all species: Winston et al. 2007, Nuez & Félix 2012. Efficiency also varies between tissues, including the germ line, even in C. elegans: Tijsterman et al. 2002; Pollard & Rockman 2013.
