Last news on SAMPLING

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The diversity of Caenorhabditis is just starting to be uncovered, and we likely have only scratched the surface. And remember that the cash and naming prize for the C. elegans sister species is - unfortunately - still not claimed. So please SAMPLE and share! For methods, see: WormMethod chapter by Barrière and Félix, Andersen lab.

Félix lab

The fall 2013 was very rainy and busy, with sampling of lots of C. elegans, C. briggsae and associated organisms from France by Lise Frézal, Sarah Marsh and MAF. Be prepared to pronounce "Crouy-sur-Ourcq" as a new location. The Orsay virus was finally found again by Lise in Ivry near Paris. Sarah established a collection of bacteria isolated from C. elegans and C. briggsae, presumably most from their guts. Marie-Anne isolated an oomycete infecting C. elegans, identified by 18S as a Myzocytiopsis sp. (for those who like her have no clue what oomycetes are, they are related to brown algae and diatoms).

And thanks to many samplers, in the last year we isolated:

- a new species C. sp. 31 from Peru, with a beautiful male tail, as well as some C. briggsae and C. elegans from Peru

- C. briggsae from Mayotte

- a sp. 9 from Sao Tome, extending the Afro-Asian equatorial distribution of this species (from W to E: Sao Tome, Congo, Seychelles, Kerala, Singapore)

- a second C. elegans isolate from Greece

- a new collection of C. elegans from the botanical garden of Lisbon, Portugal

- a sp. 11 from Costa Rica

- C. elegans from Kerala, South of India!

- C. remanei from Strasbourg ... and from near Paris (thanks to Christian Braendle's prior finding)

- and finally sp. 6 again (after Michael Ailion first found it in Portugal) near Paris and in Ireland, all in rotting apples.

Conclusion: we are far from having sampled the Paris region to saturation, finding two more species this year, adding up to five (Cel, Cbr, Cre, spp. 13 and 6). Nouragues in French Guiana still holds the species diversity record with six species in a smaller area.

Tell us if you are interested in some of these isolates (here is our current collection of isolates, not yet including the most recent, nor our collection of C. elegans kept as potential heterozygotes by freezing the whole progeny of each wild P0 individual).